The target genes ended up predicted based mostly on the attributes of large complementarity of miRNAs with the focus on gene sequence. The benefits showed that 1197 transcripts of A. konjac have been the prospective goal genes of miRNAs. Some goal genes exhibited no definite capabilities, while the annotated targets are concerned in transcriptional regulation, fat burning capacity, signal transduction, pressure goto response, digital transmission, and other daily life procedures. Handful of scientific studies on the regulation of glucomannan synthesis have been executed. By way of conducting miRNA goal gene prediction, we located that four associates of the genes participating in glucomannan synthesis are probably controlled by miRNA. These users were SS and corresponding miR339, UGP and corresponding miR156 and starch synthase III precursor and corresponding miR5763. These miRNA may carry out critical regulatory features in KGM synthesis. For example, miR339 induces the silencing of sucrose synthase mRNA by combining with the transcripts of sucrose synthase, therefore managing sucrose degradation and sucrose synthase production at a transcription degree. This method also regulates starch and glucomannan synthesis. In the leaves, fructose and glucose can be acquired via sucrose decomposition and also can be right developed by photosynthesis, delivering raw components of glucomannan and starch synthesis. Nonetheless, fructose and glucose in the corms of Amorphophallus are mostly acquired from sucrose decomposition. For that reason, the suppression of sucrose synthase in the leaves perhaps increased the use of photosynthesis-developed glucose and fructose in starch and glucomannan synthesis. The carbs in Amorphophallus incorporate fairly intricate substances this kind of as glucose, fructose, starch, sucrose, and glucomannan. Among these substances, fructose and glucose are synthesized via photosynthesis in daytime or attained from sucrose degradation, in which sucrose is converted to produce starch or glucomannan and other polysaccharides. So, the pathway of glucomannan biosynthesis was relevant to sucrose metabolism, nucleotide sugar conversion pathways. Six of these enzymes, namely, sucrose synthase, phosphoglucose isomerase, phosphoglucomutase, phosphomannose isomerase, phosphomannomutase, and starch synthase are found in Amorphophallus. Experimental proof has also demonstrated that these enzymes exhibit corresponding catalytic features. In addition, AkCSLA3 gene was cloned from A. konjac and the enzyme was verified possessing the glucomannan mannosyl- and glucosyl transferase pursuits. The framework and phylogeny of CSLD proteins have led to tips that the proteins would be glucan synthases utilizing UDP-glucose as a substrate and preliminary research has indicated that CSLD proteins are also glucomannan synthases. Taking into consideration the annotation benefits of the transcriptome data and the reported final results, the possible biosynthetic pathway of KGM and starch in konjac leaf was built. Amid these transcripts, the mRNA sequences of fructokinase and cellulose synthase-like D have been described for the 1st time, indicating that the corresponding genes of the two enzymes were current in A. konjac and A. bulbifer. However, enzyme routines need to be verified by conducting additional investigations. In the identified glucomannan biosynthesis pathways, only GDP-D-pyrophosphorylase is absent in the leaves of Amorphophallus. And GGP is also not found in konjac corms. Heller et al. discovered UDP-glucose, ADP-glucose and GDP-mannose in konjac corms, but no GDP-glucose. It would seem there is tiny likelihood of the glucose units in KGMs acquired from GDP-glucose. The feasible way of KGM synthesis might be GDP-mannose and UDP-glucose was catalyzed by CSLD proteins. This deduction requirements the further experiments. In the expressed genes, the quantities of the corresponding transcripts of various useful genes differed drastically. For case in point, PGI exhibited only one kind of transcript with a considerably higher expression stage in A. bulbifier than in A. konjac. PMI confirmed only two sorts of transcripts with a considerably lower expression level in A. bulbifier than in A. konjac. As GDP-mannose is synthesized from mannose-one-phosphate, two types of GDP-mannose pyrophosphorylase are involved primarily based on the substrate sort: type I utilizes GTP and mannose-1-phosphate as the substrates and variety II utilizes GDP and mannose-one-phosphate as the substrates. In this review, the corresponding transcripts of each sorts of enzymes had been present in Amorphophallus leaves, but type II uncovered only one transcript with a reduced expression, indicating that GMPP type I was the principal enzyme concerned in the catalytic synthesis of GDP-mannose. The starch is classified into amylose and amylopectin. The synthesis of plant amylose is catalyzed by granule-certain starch synthase amylopectin synthesis can be synergistically catalyzed by soluble starch synthase, starch branching enzyme, and debranching enzyme. The corresponding transcripts of these 4 enzymes localized in the chloroplasts have been identified in the transcriptome of Amorphophallus. GBSS exhibited much less transcripts but higher expression ranges. SSS and SBE confirmed larger quantities of transcripts. Significant differences in expression amounts have been observed among a variety of transcripts. DBE comprised only two transcripts with relatively lower expression ranges. The KGM material is an crucial indicator of top quality. As a result, the starch content of Amorphophallus affects the glucomannan content material. In glucomannan and starch synthesis pathway, glucose-one-phosphate is catalyzed to synthesize GDPglucose, ADP-glucose or DUP-glucose, which participate in glucomannan synthesis or starch synthesis, respectively. But no evidence uncovered GGP current in konjac. As a result, UGP and AGP are the important enzymes utilised to determine the in vivo synthesis of glucomannan and starch in Amorphophallus.