DZ-9-47 (109, 1010, 1011 and 1012 get Tubacin spores per plant) have been compared at 90 days immediately after transplant. When compared with the untreated manage, application with 1012 spores of HDZ-7-24 per plant lowered the galling indices by 52-70 . In one more test, combination of bio-fumigation soil remedy applying cabbage residues followed by application of 1012 spores of HDZ-9-47 reduced the galling indices by 81-88 . It was larger than these of HDZ-9-47 (5270 ) or bio-fumigation (46-58 ) alone, and was not significantly distinct in the fosthiazate (68-80 ). Furthermore, HDZ-9-47 against M. incognita on cucumber was evaluated for 3 successive developing seasons from 2010 to 2011. The results showed that mixture of bio-fumigation soil therapy using cabbage residues and application of 1012 spores of HDZ-9-47 per plant lowered the galling indices by 48-53 at 90 days after transplant, which was equal to that of fosthiazate (50 ). Our benefits demonstrated that soil pre-treatment with bio-fumigation followed by drench application of ferment filtrate of HDZ-9-47 had synergistic effects against RKN in field. S. rubrogriseus HDZ-9-47 was a promising agent forMeeting Abstracts 499 biological handle of RKN and future researches ought to concentrate on the identification of nematicidal compounds, optimization of fermentation technologies and commercial formulation of S. rubrogriseus. MOLECULAR CHARACTERIZATION OF MELOIDOGYNE INCOGNITA FROM A PEACH ORCHARD IN NORTH CAROLINA. Ye, Weimin1, David Dycus1, and Steve Koenning2. 1Nematode Assay Section, Agronomic Division, North Carolina Department of Agriculture Customer Services, Raleigh, NC 27607; and 2Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695. A 2012 survey of root-knot nematodes inside a peach orchard in Moore County, NC, revealed serious root-knot nematode infection in some rows of trees of unknown rootstock. Trees with Guardian rootstock, which protects trees from peach tree brief life illness, have been healthy. Second-stage juveniles in samples taken about infected trees numbered 3,590 per 500 cc soil. The species was identified as Meloidogyne incognita by means of PCR and DNA sequencing around the near-full-length compact subunit rDNA gene, D2/D3 expansion segments in the huge subunit rDNA gene, internal transcribed spacer, intergeneric spacer, mitochondrial DNA cytochrome oxidase subunit II and the 16S rRNA gene, histone gene and an uncharacterized protein coding gene. Species-specific molecular primers and probes had been created to recognize M. incognita depending on the histone gene and were applied in real-time PCR assay for fast species identification. OCCURRENCE OF PASTEURIA SPP. ATTACKING Different NEMATODES IN PASTURES OF BINGOL, TURKEY. Yildiz, Senol1 and Z. A. Handoo2. 1Department of Horticulture, Michigan State University, East Lansing, MI, 48824; and 2 USDA-ARS Nematology Laboratory, Beltsville, MD, 20705. In spring 2011, a survey of plant-parasitic nematodes in pastures was performed in Bingol Province, a hugely mountainous and temperate location in Eastern Anatolia. The province relies on pastures to support its livestock market, which can be by far the most essential driving force from the economy. A total of 24 samples had been collected from four districts (Bingol, Genc, Karliova and Solhan) inside the province. Nematodes have been extracted and fixed in TAF, mounted on permanent slides, and identified.