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  • Ewing Bennett posted an update 8 years, 5 months ago

    Baker Center for Plant Breeding, Iowa State University, Ames, IA. Amongst the pathogenic species of nematodes threatening soybean (Glycine max) production, the soybean cyst nematode (Heterodera glycines; SCN) will be the most damaging. The release of cultivars resistant to SCN is as a result of utmost importance, since genetic resistance could be the most effective mode to control the pathogen and defend soybean yield. Classical breeding approaches have successfully delivered resistant cultivars and continue to do it. Even so, molecular tools applied to each BIM-22493 biological entities, soybean and nematodes, have opened up possibilities for escalating breeding efficiency. This presentation will address the present use of technologies. It can also address how the advent of new molecular info and technologies might additional contribute to improve breeding efficiency in soybean for resistance for the soybean cyst nematode. NEW SPECIES OF CALOOSIA, CRICONEMA, HEMICYCLIOPHORA AND MESOCRICONEMA (NEMATODA: CRICONEMATOIDEA). Cordero, Marco and R.T. Robbins. Plant Pathology Division, 2601 N. Young Ave. CralleyWarren Analysis Lab. University of Arkansas, Fayetteville, AR. 72704. New species of Criconematoidea found in Arkansas and North Carolina amongst 2008-2011 are reported. Nematodes have been killed and fixed in hot three formaldehyde, and subsequently infiltrated with glycerin using Seinhorst’s modified slow method. Morphological characterization was performed by acquiring the nematode’s morphometrics represented by measurements and ratios applying light microscopy (Nikon Optophot-2) as well as a Nikon Drawing Tube. Caloosia n. sp. was found associated with pines and Paspalum sp. is characterized by getting a extended physique (1081.3-1431.3 mm), long stylet (105.6-123.8 mm), flattened physique annuli (R=338-357), interruptions in physique annuli laterally that demarcate a single lateral field with out lines along with a very simple rounded vulva slit (V=75.8-77.8), without having modified vulva lips. Criconema n. sp. 1 was identified related with hackberry, Paspalum sp., maple and oatgrass. The species includes a conspicuous head offset from the body with two annuli, short roundedMeeting Abstracts 459 tail with a thin cuticular sheath and subterminal anus. Criconema n. sp. two was located linked with Paspalum sp. Specimens have two cephalic annuli regarding the very same width, 1st annulus directed posteriorly, separated by a narrow neck annulus and a short conoid tail with a unilobed non-folded annulus. Hemicycliophora n. sp. was found related with turfgrass in North Carolina and is characterized by obtaining a lateral field demarked by two faint lines with transverse anastomoses and/or breaks on the striae, lengthy vulva lips and vulval sleeve, an elongated, non offset, conical tail, with distinct annulations ending within a rounded tip. Mesocriconema n. sp. was found related with Paspalum spp. and is characterized by obtaining small flattened submedian lobes, reduce than labial disc or at the similar level, vagina straight, extremely effectively created rounded spermatheca with out sperm, no a lot more than a single anastomoses, L= 379-512 mm, V=88.7-92.9, stylet length = 48.7-60.9 mm, R=107-119, annuli with crenate margins at tail portion and a basic anterior vulva lip. ITS-1 amplicons were obtained and submitted to GenBank. The genus Caloosia has been reported in India, Bangladesh, Sri Lanka, South Africa, Fiji, and Hawaii islands. This can be the initial report in the genus in North America. PREPARE YOURSE.