D TX-2076 had high SB-742457 levels of resistance whereas TX-2107 only had moderate levels of resistance. Resistance in TX-1174 and TX-2076 appears to be governed by two dominantly inherited genes whereas TX-2107 had resistance governed by one dominant gene. The objective of this study is usually to recognize and map root-knot nematode resistance loci in these special G. hirsutum lines (TX-1174, TX-2107, and TX-2076) using simple sequence repeat (SSR) markers. The lines used are going to be: 1) inoculated with M. incognita, 2) phenotypically analyzed by measuring the nematode reproduction as eggs per gram of fresh root and host response working with a root gall index, three) genetically evaluated by using SSR markers to detect polymorphisms in between the 3 RKN resistant TX lines and DP90 (susceptible line), and four) analyzed utilizing linkage and mapping software. Genotypic analysis of those lines that would further help the half-diallele study is at present underway. To date, 48 in the 150 SSR markers that have been tested have shown polymorphisms involving the three RKN resistant TX lines and the susceptible DP90. A bulked segregant evaluation approach is becoming used to test resistant and susceptible bulks of the F2 population. The rest with the F2 population are going to be tested when the SSR markers continue to indicate polymorphisms among the bulks. Identification of SSR markers linked to RKN resistance will facilitate marker-assisted choice in breeding programs exactly where the objective will be to develop new cultivars that contain RKN resistance. HOST-SEEKING, OLFACTION, FORAGING Strategies, And the GENOMIC ARCHITECTURE OF PARASITISIM Amongst STEINERNEMA NEMATODES. Dillman1, Adler, Ali Mortazavi2, Elissa Hallem3, and Paul W. Sternberg1. 1Howard Hughes Medical Institute, Division of Biology, California Institute of Technology, Pasadena, CA 91125; 2Developmental and Cell Biology, Center for Complicated Biological Systems, University of California, Irvine, Irvine, CA 92697; and 3Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095. Several parasitic nematodes actively seek out hosts to infect and hence full their lifecycles. Olfaction is believed to play a vital role in the host-seeking method, with parasites following a chemical trail toward host-associated odors. Steinernema can be a diverse genus of entomopathogenic nematodes, with more than 60 described species. The host-seeking behaviors of Steinernema consist of chemotaxis and jumping behavior and differ along a foraging method continuum amongst cruise and ambush methods. Small is identified about the odors that stimulate host-seeking behavior or how olfactory facts is interpreted in these agriculturally crucial parasitic nematodes. We explored the host-seeking responses of a number of Steinernema species to CO2 and volatile organic compounds created by laboratory and ecologically relevant potential hosts. We show that all Steinernema species tested are attracted to CO2, but display varying behavioral responses to different prospective hosts, suggesting that they can differentiate amongst potential hosts determined by odor alone. CO2 seems to play a important function in the host-seeking approach for steinernematids, though this varies for distinctive parasite-host combinations and for diverse host-seeking behaviors. Further, we utilized gas chromatography-mass spectrometry and solid-phase microextractiongas chromatography-mass spectrometry to determine odorants emitted by seven possible hosts. We found.