Protein content material from each fractions was measured with a Bradford assay. Fraction 1 contained 42.5 mg/mL protein, whereas 1:ten dilution of fraction 2 contained 60.4 mg/mL protein. Both fractions had been subjected to polyacrylamide gel electrophoresis below denaturing situations (SDS-PAGE) and many peptide bands ranging in size from ten kDa to >100kDa have been observed. Metabolite profiling of each fractions with high overall performance liquid chromatography (HPLC) / mass spectrometry (MS) revealed numerous sugars, fatty acids and also other metabolites of interest. Lots of of the metabolites happen to be reported to have or kind part of compounds with antimicrobial activity, for instance adipic acid, butylamine, hydroquinone, and lactic acid. Additionally, when we plated GMs in nutrient agar with bacteria (e.g., Xanthomonas spp., Escherichia coli), inhibition of bacterial growth was observed as a clear area surrounding the GMs suggesting that the GMs exhibited antimicrobial activity. This may confer protection for the eggs against microorganisms, and may possibly limit the possible for biocontrol organisms to handle H. glycines. EFFICACY OF MULTIGUARD PROTECTEC AT Escalating SOIL DEPTHS IN TURFGRASS. Luc, John E. and William T. Crow. Entomology and Nematology Department, University of Florida, PO Box 110620 Natural Region, Dr., Gainesville, FL 32611-0620. Multiguard ProtectEC, a commercial formulation of furfural, can be a fairly new nematicide for turfgrass. Laboratory, greenhouse, and field Title Loaded From File trials in other crops have made encouraging nematicidal final results; on the other hand turf field trials have hadMeeting Abstracts 475 inconsistent final results. Field trials were performed to determine the impact of application system around the effectiveness of Multiguard ProtectEC at rising depths with the soil profile in turf. Treatment options consisted of: i)Multiguard ProtectEC 75L/ha with 0.64-cm of irrigation applied ahead of and after application, ii) Multiguard ProtectEC 75L/ha followed by an added 75L/ha applied immediately after 1 hour with 0.64-cm of irrigation following each and every application, and untreated control receiving 1.28-cm of irrigation. Treatment options were applied 3 occasions at two-week intervals to turf plots. The experimental style was a randomized comprehensive block with five replications and carried out at two places naturally infested with Belonolaimus longicaudatus. Nematode samples consisted of eight cores (three.5-cm-diamter 3 20-cm-depth) taken from each and every plot that were then cut into depth sections following removing the best 5-cm of turf and thatch. Soil depths observed had been 0-5 cm, 5-10 cm, and 10-15 cm. Nematode and % green cover data were collected to decide pretreatment levels, after which collected two, 4, and 6 weeks immediately after initial application. Root samples were collected pretreatment and 8 weeks later. Nematode reductions had been observed between Multiguard ProtectEC and untreated handle at quite a few depths and dates through each trials. Multiguard ProtectEC 75L/ha + 75L/ha provided one of the most constant nematode reductions. Additionally, nematode reductions had been most constant in the two deepest soil depths. Variations in % green cover were observed, but have been inconsistent across trails. No differences amongst Multiguard ProtectEC and untreated manage were observed through either trial for total root length. The lack of nematicidal impact within the top rated five cm of the soil profile could be resulting from chemical conversion, interaction with organic matter or chemical reacti.