The concentrate on genes have been predicted dependent on the qualities of large complementarity of miRNAs with the target gene sequence. The benefits confirmed that 1197 transcripts of A. konjac ended up the potential target genes of miRNAs. Some goal genes exhibited no definite capabilities, whilst the annotated targets are included in transcriptional regulation, metabolic process, sign transduction, anxiety response, electronic transmission, and other lifestyle procedures. Number of scientific studies on the regulation of glucomannan synthesis have been carried out. By means of conducting miRNA concentrate on gene prediction, we discovered that 4 customers of the genes collaborating in glucomannan synthesis are potentially regulated by miRNA. These members were SS and corresponding miR339, UGP and corresponding miR156 and starch synthase III precursor and corresponding miR5763. These miRNA may complete critical regulatory capabilities in KGM synthesis. For illustration, miR339 induces the silencing of sucrose synthase mRNA by combining with the transcripts of sucrose synthase, thus managing sucrose degradation and sucrose synthase production at a transcription level. This process also regulates starch and glucomannan synthesis. In the leaves, fructose and glucose can be obtained through sucrose decomposition and also can be right made by photosynthesis, supplying raw materials of glucomannan and starch synthesis. Nevertheless, fructose and glucose in the corms of Amorphophallus are mainly obtained from sucrose decomposition. Consequently, the suppression of sucrose synthase in the leaves perhaps increased the use of photosynthesis-produced glucose and fructose in starch and glucomannan synthesis. The carbs in Amorphophallus have relatively sophisticated substances this sort of as glucose, fructose, starch, sucrose, and glucomannan. Among these substances, fructose and glucose are synthesized by means of photosynthesis in daytime or obtained from sucrose degradation, in which sucrose is transformed to make starch or glucomannan and other polysaccharides. So, the pathway of glucomannan biosynthesis was associated to sucrose fat burning capacity, nucleotide sugar conversion pathways. Six of these enzymes, namely, sucrose synthase, phosphoglucose isomerase, phosphoglucomutase, phosphomannose isomerase, phosphomannomutase, and starch synthase are discovered in Amorphophallus. Experimental evidence has also revealed that these enzymes exhibit corresponding catalytic capabilities. In addition, AkCSLA3 gene was cloned from A. konjac and the enzyme was confirmed possessing the glucomannan mannosyl- and glucosyl transferase activities. The construction and phylogeny of CSLD proteins have led to recommendations that the proteins would be glucan synthases utilizing UDP-glucose as a substrate and preliminary analysis has indicated that CSLD proteins are also glucomannan synthases. Considering the annotation results of the transcriptome info and the documented outcomes, the achievable biosynthetic pathway of KGM and starch in konjac leaf was made. Between these transcripts, the mRNA sequences of fructokinase and cellulose synthase-like D have been noted for the initial time, indicating that the corresponding genes of the two enzymes had been current in A. konjac and A. bulbifer. However, enzyme activities should be confirmed by conducting even more investigations. In the known glucomannan biosynthesis pathways, only GDP-D-pyrophosphorylase is absent in the leaves of Amorphophallus. And GGP is also not found in konjac corms. Heller et al. discovered UDP-glucose, ADP-glucose and GDP-mannose in konjac corms, but no GDP-glucose. It appears there is tiny likelihood of the glucose models in KGMs obtained from GDP-glucose. The attainable way of KGM synthesis may well be GDP-mannose and UDP-glucose was catalyzed by CSLD proteins. This deduction requirements the additional experiments. In the expressed genes, the quantities of the corresponding transcripts of numerous practical genes differed substantially. For illustration, PGI exhibited only 1 sort of transcript with a significantly higher expression stage in A. bulbifier than in A. konjac. PMI confirmed only two sorts of transcripts with a substantially decrease expression degree in A. bulbifier than in A. konjac. As GDP-mannose is synthesized from mannose-one-phosphate, two kinds of GDP-mannose pyrophosphorylase are associated primarily based on the substrate sort: kind I employs GTP and mannose-1-phosphate as the substrates and variety II makes use of GDP and mannose-one-phosphate as the substrates. In this review, the corresponding transcripts of equally types of enzymes had been current in Amorphophallus leaves, but variety II unveiled only one particular transcript with a reduced expression, indicating that GMPP kind I was the major enzyme associated in the catalytic synthesis of GDP-mannose. The starch is classified into amylose and amylopectin. The synthesis of plant amylose is catalyzed by granule-sure starch synthase amylopectin synthesis can be synergistically catalyzed by soluble starch synthase, starch branching enzyme, and debranching enzyme. The corresponding transcripts of these four enzymes localized in the chloroplasts were found in the transcriptome of Amorphophallus. GBSS exhibited less transcripts but larger expression amounts. SSS and SBE confirmed larger numbers of transcripts. Substantial distinctions in expression stages had been observed among different transcripts. DBE comprised only two transcripts with comparatively reduced expression stages. The KGM content material is an important indicator of quality. As a result, the starch content material of Amorphophallus has an effect on the glucomannan material. In glucomannan and starch synthesis pathway, glucose-1-phosphate is catalyzed to synthesize GDPglucose, ADP-glucose or DUP-glucose, which take part in glucomannan synthesis or starch synthesis, respectively. But no proof uncovered GGP current in konjac. Consequently, UGP and AGP are the important enzymes utilised to determine the in vivo synthesis of glucomannan and starch in Amorphophallus.