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  • Ewing Bennett posted an update 6 years, 6 months ago

    In Brazil, the breeding program of widespread bean has been directed for the establishment of upright cultivars as a result of the growing demand, within the final years, for plants which can be very easily harvested mechanically. This work dealt together with the assessment of some Brazilian frequent bean upright cultivars, which could sooner or later be rated as sources of genes454 Journal of Nematology, Volume 44, No. 4, December 2012 for nematode resistance, in relation to M. incognita and P. brachyurus. Inside a trial referred to as Experiment 1, twenty cultivars (BRS Campeiro, FT Soberano, IPR Chopim, IPR 139, BRS Estilo, BRS Radiante, IAPAR 81, IPR Tuiuiu, BRS Xamego, IPR Uirapuru, IPR Corujinha, IPR Juriti, BRS Agreste, IPR Grauna, IPR Tiziu, BRS Esplendor, IPR Gralha, IPR Tangara, IAC Diplomata and BRS Valente) had been inoculated (Pi) with 300 M. incognita (eggs+J2)/plant and the susceptible controls were SGC707 site cotton `Fibermax 966′ and maize `DKB 330′. Within the experiment 2, nine cultivars (BRS Valente, BRS Campeiro, IPR Tuiuiu, IPR 139, FT Soberano, IPR Chopim, BRS Estilo, BRS Radiante and IAPAR 81) had been inoculated with 300 P. brachyurus (eggs + motile forms)/plant along with the susceptible controls had been soybean `Pintado’ and popular bean `Perola’. In both trials, the experimental design and style was absolutely randomized with six replications and they extended for 60 days till final evaluation. Data of final population (Pf), nematodes/ g of roots (Nem/g) and nematode reproduction factor (RF = Pf/Pi) had been recorded. Inside the experiment 1, each of the cultivars were rated as susceptible to M. incognita (RF 1). Among the typical bean cultivars, IPR Corujinha showed the lowest RF (five.86) and Nem/g (158) values. The Rf values of the cotton `Fibermax 966′ as well as the maize `DKB 330′ have been 8.05 and 2.70, respectively. Inside the experiment 2, all of the cultivars have been susceptible to P. brachyurus. The cultivars BRS Valente and BRS Campeiro showed the lesser RF (five.26 and five.38, respectively) and Nem/g (142 and 138, respectively) values. The RF values determined for the soybean `Pintado’ and frequent bean `Perola’ were of 4.25 and 14.88, respectively. From these results it was inferred that no source of resistance to the nematodes tested was detected amongst all of the prevalent bean upright cultivars assessed. AN EFFECTOR D26 May possibly BE INVOLVED In the SUPPRESSION OF PLANT DEFENSE Via THE INTERACTION WITH FTR-C. Borong, Lin, K. Zhuo, and J.L. Liao. Laboratory of Plant Nematology, South China Agricultural University, Guangzhou, China 510642. Meloidogyne is one group of vital plant pathogenic nematodes, causing considerable losses of a lot of crops within the planet. Some worthwhile research have already been conducted to investigate the molecular mechanism involving in nematode infection. Reactive oxygen species (ROS), specifically hydrogen peroxide (H2O2), has been verified to be related for the expression of defense-related genes by the nematode infection in the nematode-plant interaction. A gene D26 encoding for a 125-aa protein using a predicted molecular mass of 14.3 KDa was isolated from M. javanica. The effector has been shown to become a secreted protein from esophageal gland. Over expression of D26 rendered Arabidopsis susceptible to M. javanica. The yeast two hybrid screening and BIFC had been carried out to indicate that the protein interacts with FTR-c in Arabidopsis thaliana.